1. The kit is based on isothermal nucleic acid amplification using oligonucleotide primers, a combination of three enzymes and amplicon specific probe for fluorogenic real-time detection. The specific primers and probe are IPR of Nextec Lifesciences.

2. Detects Mycobacterium tuberculosis (TB bacteria) specific DNA in clinical fluid of suspected TB patients.

Rapid and clinician friendly  - Results available online within 10 min

Allows detection of early infection (sensitive) - Detects as low as 10 TB bacilli

Specificity - Test is 100% specific when tested on clinical samples from suspected TB patients, using culture as gold standard. No false positives were detected

Multiplex - Two M. tb specific genes (targets) can be detected in a single reaction in a single tube (multiplex). Amplification of two genes is detected by fluorometry using two fluorophores/ agarose gel electrophoresis.

References - 

 1.  R. Srivastava, D. Kumar, M.N. Waskar, M. Sharma, V.M. Katoch, B.S. Srivastava. 2006. Identification of a repetitive sequence belonging to a PPE gene of Mycobacterium tuberculosis and its use in diagnosis of tuberculosis .J. Med. Microbiol. 55, 1071.

 

2. Mycobacterium tuberculosisspecific  DNA  fragment. Ranjana  Srivastava, Deepak Kumar, B.S. Srivastava. US Patent no. 6,242,585 B1, 5. 6. 2001.

 

3.  Mycobacterium tuberculosisspecific  DNA  fragment. Ranjana Srivastava, Deepak Kumar, B.S. Srivastava. US Patent no. 6114514, 05. 09. 2000 

 

4..Oligonucleotide primers useful for detection of mycobacterium species"    Ranjana Srivastava, Deepak Kumar, Brahm S. Srivastava   Indian Patent no. 244289 29.11. 2010

 

5. A Kit and assay method for detection of Mycobacterium tuberculosis complex. Ranjana  Srivastava, B.S. Srivastava.  Indian Patent 355147 , 25.05.2017